English synonyms: beta-Nicotinamide adenine dinucleotide trihydrate nadide Coenzyme IDiphosphopyridine dinucleotidebeta-Diphosphopyridine nucleotide cozymase beta-NADNAD beta-dpnbeta Diphosphopyridine nucleotide;Cozymase Hydrate,oxidized form;β-Nicotinamide adenine dinucleotide hydrate;β-Diphosphopyridine Nucleotide Hydrate,oxidized form;β-Nicotinamide adenine dinucleotide;Coenzyme I Hydrate,oxidized form;β-DPN Hydrate,oxidized form;beta-Diphosphopyrid;β-NAD Hydrate,oxidized form;Diphosphopyridine nucleotide;β-Nicotinamide adenine dinucleotidebeta-Nicotinamide Adenine Dinucleotide Hydrate, oxidized form[for Biochemical Research]β-NADNAD TRIHYDRATE
Physicochemical properties of coenzyme I
Nicotinamide adenine dinucleotide (or nicotinamide adenine dinucleotide, or NAD), formerly known as pyridine diphosphate (DPN) or secondary dehydrogenation Enzyme I or Coenzyme I. It is a coenzyme that transfers electrons (more precisely: hydrogen ions), which occurs in many metabolic reactions of cells. NADH or more accurate NADHH is its reduced form.
It can be restored with up to two electrons (written as NADHH).
NAD is a coenzyme of dehydrogenase, such as alcohol dehydrogenase (ADH), used to oxidize ethanol. It plays an irreplaceable role in glycolysis, gluconeogenesis, the Krebs cycle and the respiratory chain. The intermediate product will pass the desorbed hydrogen to the NAD to make it NADH.
NADH is used as a carrier of hydrogen to synthesize ATP by chemical osmosis coupling in the respiratory chain.
In terms of light absorption, NADH has an absorption peak at 260 nm and 340 nm, and NAD has only one absorption peak at 260 nm, which is an important attribute for distinguishing both. This is also the physical basis for measuring metabolic rates in many metabolic tests. The absorption coefficient of NAD at 260 nm is 1.78 × 10 L / (mol · cm), and the absorption coefficient of NADH at 340 nm is 6.2 × 10 L / (mol · cm).
NAD+ can participate in glycolysis:
C6H1206+2NAD+ +2ADP+2pi——2CH3COCOOH+2NADH+2H+ +2ATP
The molecular weight is: 663.4
Use of coenzyme I
Because NAD is a coenzyme of a large number of oxidoreductases in the human body, it can also be used as a direct template for drug design, or indirectly based on the structure of NAD to design enzyme inhibitors or agonists to alter the activity of NAD-acting enzymes, or directly To inhibit the biosynthesis of NAD.
NAD may be an important anti-aging drug in the future.
Participation in energy metabolism and material metabolism in the body is conducive to cell repair and renewal. For the treatment of coronary heart disease, myocarditis, leukopenia and other diseases.
Coenzyme I production method
The yeast is extracted by boiling water, and the lead acid is acidified and precipitated to obtain a crude product, which is obtained by subjecting a formic acid type cation exchange resin column to purification.
Upstream and downstream product information
The only one in China, only Nutra Biotech Co.,Ltd successfully developed enzymatic preparation of NAD
Modern biocatalytic technology mimics the reaction of enzymes in vivo. This simulation condition is based on the metabolic characteristics of the organism, leucine dehydrogenase, formate dehydrogenase, glucose dehydrogenase. All need the help of NAD to complete the whole reaction.
NAD is one of the coenzymes essential for modern biocatalytic reactions.
The popular Sir2 protein for research is a deacetylase that relies on NAD, and its role is to extend the cell’s life cycle (anti-aging).